One-Carbon Metabolism, a Nutrient Dependent Mediator of Angiogenesis and Alveolar Formation in the Developing Lung

Background/Objective: Lung maturation has discrete dynamic shifts in metabolic derivatives that give rise to stage specific metabolite signatures. Components of one-carbon metabolism (OCM) are significantly increased the late saccular and alveolar while hyperoxia disrupts OCM during these lung developmental stages. We hypothesize disrupted metabolites immature vessel dependent formation thus contributing dysplasia. Our studies explore impact restoration on angiogenesis formation.Methods: Isolated human neonatal circulating Endothelial Colony Forming Cells (ECFCs) were examined for proliferation (WST-1), migration (scratch assay), (matrigel) response supplementation derivatives, essential amino acid methionine nicotinamide. enzyme levels assessed via Western blotting, NNMT, NAMPT, DNMT. Lastly, a murine model prematurity (HMMP) with dietary supplement vs control was assessed, MLI, H&E, (endomucin).Results: Methionine nicotinamide ECFC (p<0.05) enzymes. Nicotinamide (p<0.05). not impacted by or (p NS). HMMP improved distal formation.Conclusion Clinical Implications: is shifting needs development. Replenishment key promotes formation. Future necessary role precision nutritive development premature infants.